Cell Signaling Technology

Product Pathways - Translational Control

DDX4 (D10C5) Rabbit mAb #8761

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F H M (Mk) Endogenous 80 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DDX4 (D10C5) Rabbit mAb recognizes endogenous levels of total DDX4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human DDX4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse testes and brain using DDX4 (D10C5) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

IF-F

IF-F

Confocal immunofluorescent analysis of mouse testes (left) and mouse brain (right) using DDX4 (D10C5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

DDX4 is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells (1). This enzyme is specific to germ cells and is necessary for germ cell development (2). Mouse DDX4 was shown to interact with Dicer, suggesting a role in microRNA-mediated RNA silencing (1).

  1. Kotaja, N. et al. (2006) Proc Natl Acad Sci USA 103, 2647-52.
  2. Albamonte, M.S. et al. (2008) Hum Reprod 23, 1895-901.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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