Cell Signaling Technology

Product Pathways - Autophagy Signaling

Atg101 Antibody #8764

Applications Reactivity Sensitivity MW (kDa) Source
W H M Endogenous 25 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Atg101 Antibody recognizes endogenous levels of total Atg101 protein. This antibody also detects a 55 kDa protein of unknown origin in some cell lines.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val177 of human Atg101 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Atg101 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human Atg101 (hAtg101-Myc/DDK; +), using Atg101 Antibody (upper) or Myc-Tag (71D10) Rabbit mAb #2278 (lower).

Background

Atg101 was discovered as a binding protein for Atg13, a component of the ULK1 serine-threonine kinase required for autophagy (1-3). Autophagy is a catabolic process for the autophagosomic-lysosomal degradation of bulk cytoplasmic contents (4,5). It is generally activated by conditions of nutrient deprivation, but is also associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer (6). The molecular machinery of autophagy was largely discovered in yeast and is directed by a number of autophagy-related (Atg) genes. The ULK1 complex includes both Atg13 and FIP200 and is required for starvation-induced autophagy (7-9). Interaction between Atg101 and Atg13 can be important for the stability and basal phosphorylation of Atg13 and ULK1 (1,2).

  1. Mercer, C.A. et al. (2009) Autophagy 5, 649-62.
  2. Hosokawa, N. et al. (2009) Autophagy 5, 973-9.
  3. Chan, E.Y. et al. (2007) J Biol Chem 282, 25464-74.
  4. Reggiori, F. and Klionsky, D.J. (2002) Eukaryot Cell 1, 11-21.
  5. Codogno, P. and Meijer, A.J. (2005) Cell Death Differ 12 Suppl 2, 1509-18.
  6. Levine, B. and Yuan, J. (2005) J Clin Invest 115, 2679-88.
  7. Hosokawa, N. et al. (2009) Mol Biol Cell 20, 1981-91.
  8. Jung, C.H. et al. (2009) Mol Biol Cell 20, 1992-2003.
  9. Ganley, I.G. et al. (2009) J Biol Chem 284, 12297-305.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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