Cell Signaling Technology

Product Pathways - Development

KIBRA Antibody #8774

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Mk Endogenous 135 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

KIBRA Antibody recognizes endogenous levels of total KIBRA protein. Higher molecular weight bands detected by western blot are phosphorylated forms of KIBRA.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala585 of human KIBRA protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using KIBRA Antibody.

Background

The Hippo pathway is an important evolutionarily conserved signaling pathway that controls organ size and tumor suppression by inhibiting cell proliferation and promoting apoptosis (1,2). An integral function of the Hippo pathway is to repress the activity of YAP (Yes-associated protein), a proposed oncogene whose activity is regulated by phosphorylation and subcellular localization (3,4). Recent studies have identified KIBRA as a novel regulator of Hippo signaling (5-7). KIBRA has been shown to regulate Hippo signaling through its interaction with tumor suppressors Merlin (Mer) and Expanded (Ex) in Drosophila (7) and by associating with large tumor suppressors LATS1 and LATS2 in humans (8). In humans, KIBRA is predominantly expressed in the kidney and brain (9) and has been shown to play a role in hippocampus-related memory performance (10-12). Recent studies have shown that phosphorylation of KIBRA is highest during mitosis and is controlled by aurora kinase and protein phosphatase 1 (13).

  1. Pan, D. (2010) Dev Cell 19, 491-505.
  2. Harvey, K.F. et al. (2003) Cell 114, 457-67.
  3. Zhao, B. et al. (2010) Genes Dev 24, 862-74.
  4. Zhao, B. et al. (2008) Curr Opin Cell Biol 20, 638-46.
  5. Baumgartner, R. et al. (2010) Dev Cell 18, 309-16.
  6. Genevet, A. et al. (2010) Dev Cell 18, 300-8.
  7. Yu, J. et al. (2010) Dev Cell 18, 288-99.
  8. Xiao, L. et al. (2011) J Biol Chem 286, 7788-96.
  9. Kremerskothen, J. et al. (2003) Biochem Biophys Res Commun 300, 862-7.
  10. Papassotiropoulos, A. et al. (2006) Science 314, 475-8.
  11. Bates, T.C. et al. (2009) Neurosci Lett 458, 140-3.
  12. Schaper, K. et al. (2008) Neurobiol Aging 29, 1123-5.
  13. Xiao, L. et al. (2011) J Biol Chem 286, 36304-15.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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