Product Pathways - Companion Products
MitoTracker® Deep Red FM #8778
|8778S||500 µg (10 x 50 ug)||---||In Stock||---|
Already purchased this product? Write a Review.
MitoTracker® Deep Red FM is well retained after fixation allowing for further sample processing and immunostaining. Excitation: 644 nm, Emission: 665 nm, Molecular Weight: 543.58 g/mol
MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.
Directions For Use
Each vial of MitoTracker® Deep Red FM contains 50 μg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 91.98 μl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results can be obtained by diluting the stock solution directly in growth media to a final concentration of 500 nM and incubating for 30 min at 37ºC. After incubation, fix the cells in ice-cold, 100% methanol for 15 min at -20ºC and rinse 3 times with PBS for 5 min. Proceed with immunostaining.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
MitoTracker® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.