Cellular Dyes
LysoTracker® Green DND-26 #8783
Directions for Use
Each vial of LysoTracker® Green DND-26 contains 50 μl of solution at a 1 mM concentration in DMSO. Dilute 1:20,000 directly into normal growth media for a working concentration of 50 nM and analyze immediately. Cells must be imaged live; DO NOT FIX!
To reduce background fluorescence of the media, phenol-red free media can be substituted for normal media prior to imaging. A green background suppressor, such as BackDrop™ Background Suppressor from Life Technologies, can also be added to reduce background fluorescence.
IF-IC
Confocal immunofluorescent analysis of live HeLa cells, untreated (left) or chloroquine-treated (50 μM, 4 hr; right), using LysoTracker® Green DND-26 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
LysoTracker® Green DND-26 is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 504 nm, Emission: 511 nm, Molecular Weight 398.69
Background
LysoTracker® Green DND-26 is a cell permeable green dye that stains acidic compartments (lysosomes) in live cells.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. LysoTracker® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
