Cell Signaling Technology

Product Pathways - Neuroscience

DDC Antibody #8786

Applications Reactivity Sensitivity MW (kDa) Source
W M R (H) Endogenous 48 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

DDC Antibody recognizes endogenous levels of total DDC protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val33 of human DDC protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines and tissue using DDC Antibody (upper) and α/β-Tubulin Antibody #2148 (lower).

Background

L-DOPA decarboxylase (DDC) is a pyridoxal 5-phosphate (PLP)-dependent enzyme that catalyzes the decarboxylation of L-DOPA to dopamine (1) and L-5HTP to serotonin (2). By catalyzing the reaction to produce dopamine, DDC is involved in many important metabolic processes and plays a central role in the complex neuroendocrine-immune regulatory network (1). DDC is expressed in the central nervous system (3), but has also been detected in some peripheral organs such as the liver and adrenal gland, as well as leukocytes of rat and human (1). DDC is thought to be the sole enzyme responsible for the synthesis of the trace amines 2-phenylethylamine, p-tyramine, and tryptamine, which are considered to act as neuromodulators (2,4). DDC is also regarded as a general biomarker for neuroendocrine tumors (3).

  1. Zhou, Z. et al. (2011) PLoS One 6, e18596.
  2. Børglum, A.D. et al. (1999) Mol Psychiatry 4, 545-51.
  3. Kontos, C.K. et al. (2010) Br J Cancer 102, 1384-90.
  4. Ma, J.Z. et al. (2005) Hum Mol Genet 14, 1691-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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