Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

EphA3/A4/A5 (D2C11) Rabbit mAb #8793

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M (R) Endogenous 135 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

EphA3/A4/A5 (D2C11) Rabbit mAb recognizes endogenous levels of total EphA3, EphA4, and EphA5 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr779 of human EphA3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using EphA3/A4/A5 (D2C11) Rabbit mAb.

Background

The Eph receptors are the largest known family of receptor tyrosine kinases (RTKs). They can be divided into two groups based on sequence similarity and on their preference for a subset of ligands. While EphA receptors bind to a glycosylphosphatidylinositol-anchored ephrin A ligand, EphB receptors bind to ephrin B proteins that have a transmembrane and cytoplasmic domain (1,2). Research studies have shown that Eph receptors and ligands may be involved in many diseases including cancer (3). Both ephrin A and B ligands have dual functions. As RTK ligands, ephrins stimulate the kinase activity of Eph receptors and activate signaling pathways in receptor-expressing cells. The ephrin extracellular domain is sufficient for this function as long as it is clustered (4). The second function of ephrins has been described as "reverse signaling", whereby the cytoplasmic domain becomes tyrosine phosphorylated, allowing interactions with other proteins that may activate signaling pathways in the ligand-expressing cells (5).The EphA3 receptor preferentially binds ephrin-A5. This ligand-receptor interaction stimulates EphA3 signaling, regulates cell adhesion and migration, and induces cellular morphologic responses (6-8). EphA3 plays a critical role in callosal axon guidance (9), retinotectal mapping of neurons (10), as well as cardiac cell migration and differentiation (11). Investigators have shown that somatic mutations in functional domains of EphA3 are linked to lung cancer progression (12). In addition, EphA3 expression levels have been correlated with tumor angiogenesis and progression in gastric and colorectal carcinoma (13,14).

  1. Wilkinson, D.G. (2000) Int Rev Cytol 196, 177-244.
  2. Klein, R. (2001) Curr Opin Cell Biol 13, 196-203.
  3. Dodelet, V.C. and Pasquale, E.B. (2000) Oncogene 19, 5614-9.
  4. Holder, N. and Klein, R. (1999) Development 126, 2033-44.
  5. Brückner, K. et al. (1997) Science 275, 1640-3.
  6. Smith, L.M. et al. (2004) Exp Cell Res 292, 295-303.
  7. Clifford, N. et al. (2008) J Cell Biochem 105, 1250-9.
  8. Vearing, C. et al. (2005) Cancer Res 65, 6745-54.
  9. Nishikimi, M. et al. (2011) J Neurosci 31, 16251-60.
  10. Connor, R.J. et al. (1998) Dev Biol 193, 21-35.
  11. Li, Y.Y. et al. (1998) Am J Physiol 274, H331-41.
  12. Lisabeth, E.M. et al. (2012) Biochemistry 51, 1464-75.
  13. Xi, H.Q. et al. (2012) J Gastroenterol 47, 785-94.
  14. Xi, H.Q. and Zhao, P. (2011) J Clin Pathol 64, 498-503.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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