Product Pathways - Nuclear Receptor Signaling
STF-1 Antibody #8795
|W||H M (Mk) (B) (Pg) (Hr)||Endogenous||50||Rabbit|
Reactivity Key: H=Human M=Mouse Mk=Monkey B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
STF-1 Antibody recognizes endogenous levels of total STF-1 protein. This antibody does not cross-react with LRH-1/NR5A2.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu184 of human STF-1 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from NCI-H295R and MLTC-1 cells using STF-1 Antibody.
Western blot analysis of extracts from 293T cells, either mock transfected (-), transfected with a DDK-tagged cDNA expression construct encoding full-length human STF-1 (hSTF-1, +), or transfected with a Myc/DDK-tagged cDNA expression construct encoding full-length human LRH-1, isoform 2 (hLRH-1, +), using STF-1 Antibody (upper) and DYKDDDDK Tag Antibody (Binds to same epitope as Sigma's Anti-FLAG® M2 Antibody) #2368 (lower).
The orphan nuclear receptor, steroidogenic factor 1 (STF-1, also called Ad4BP), encoded by the NR5A1 gene, displays a high degree of homology to the Drosophila nuclear receptor fushi tarazu factor 1 and plays a fundamental role in the development and function of steroidogenic tissues. STF-1 is also similar in sequence to an orphan receptor cloned from mouse liver, designated LRH-1, and its human homolog, PHR-1. Although LRH-1 is derived from a separate gene and has a distinct expression profile, LRH-1 and STF-1 sequences are sufficiently similar to place them within the same subfamily of nuclear receptors, designated NR5A (1).Initially identified as a tissue-specific transcriptional regulator of cytochrome P450 steroid hydroxylases, studies of both global (2) and tissue-specific knockout mice (3-6) have demonstrated that STF-1 is required for the devlopment of adrenal glands, gonads, ventromedial hypothalamus, and for the proper functioning of pituitary gonadotropes. Indeed, humans with heterozygous mutations that render STF-1 transcriptionally inactive can present with testicular failure, ovarian failure, and adrenal insufficiency (7,8). Furthermore, dysregulation of STF-1 has been linked to diseases such as endometriosis (9) and adrenocortical carcinoma (10).Like other nuclear hormone receptors, STF-1 has a modular domain structure composed of an N-terminal zinc finger DNA-binding domain, a ligand-binding domain, a C-terminal AF-2 activation domain, and a hinge region with AF-1-like activation activity. STF-1 also contains a fushi tarazu factor 1 box or A-box, which functions as an accessory DNA binding domain (11). STF-1 is primarily phosphorylated at Ser203, which is thought to enhance its transcriptional activity by promoting complex formation with transcriptional cofactors (12). In addition to phosphorylation at Ser203, STF-1 is subject to SUMO conjugation and acetylation at ε-amino groups of target lysine residues. Whereas SUMOylation represses STF-1 function (13,14), acetylation enhances its transcriptional activity (15).
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For Research Use Only. Not For Use In Diagnostic Procedures.