His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) #8811
|8811S||400 µl (40 immunoprecipitations)||---||In Stock||---|
|8811||carrier free and custom formulation / quantity||email request|
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|IP||1:20||Transfected Only||Mouse IgG1|
Applications Key: IP=Immunoprecipitation
Specificity / Sensitivity
His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the 6xHis-tag fused to either the amino or carboxy terminus of targeted proteins in transfected cells.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a 6xHis synthetic peptide.
Immunoprecipitation of extracts from COS-7 cells transfected with a construct expressing a His-tagged protein (Tyro3-His), using Mouse IgG (Magnetic Bead Conjugate) #5873 (lane 2) or His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) (lane 3). Lane 1 is 10% input. The western blot analysis was performed using His-Tag Antibody #2365.
This Cell Signaling Technology antibody is immobilized by the covalent reaction of formylbenzamide-modified antibody with hydrazide-activated magnetic bead. His-Tag (27E8) Mouse mAb (Magnetic Bead Conjugate) is useful for immunoprecipitation of recombinant proteins containing the 6xHis epitope tag.
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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