Cell Signaling Technology

Product Pathways - Jak/Stat Pathway

Jak3 (D1H3) Rabbit mAb #8827

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC H (Mk) Endogenous 115 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Jak3 (D1H3) Rabbit mAb recognizes endogenous levels of total Jak3 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Jak3 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Jak3 (D1H3) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of KARPAS-299 cells (left) and A549 cells (right) using Jak3 (D1H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3, and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4), and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.

Jak3 is primarily expressed in hematopoietic cells and is required for immune cell function and development (6-8). It binds to the common γ subunit (γc), a shared receptor subunit also used by several cytokines including IL-2, IL-4, IL-7, IL-9, and IL-15 (9). IL-2 signaling and Stat5 activation are highly impaired by the loss of Jak3 (10,11). Jak3 is phosphorylated at multiple sites, including Tyr980 and 981 within the activation loop (12-14).

  1. Leonard, W.J. and O'Shea, J.J. (1998) Annu Rev Immunol 16, 293-322.
  2. Darnell, J.E. (1997) Science 277, 1630-5.
  3. VanderKuur, J. et al. (1995) J Biol Chem 270, 7587-93.
  4. Argetsinger, L.S. et al. (1995) J Biol Chem 270, 14685-92.
  5. Zhu, T. et al. (1998) J Biol Chem 273, 10682-9.
  6. Thomis, D.C. et al. (1995) Science 270, 794-7.
  7. Nosaka, T. et al. (1995) Science 270, 800-2.
  8. Park, S.Y. et al. (1995) Immunity 3, 771-82.
  9. Russell, S.M. et al. (1994) Science 266, 1042-5.
  10. Johnston, J.A. et al. (1994) Nature 370, 151-3.
  11. Oakes, S.A. et al. (1996) Immunity 5, 605-15.
  12. Zhou, Y.J. et al. (1997) Proc Natl Acad Sci USA 94, 13850-5.
  13. Cheng, H. et al. (2008) Mol Cell Biol 28, 2271-82.
  14. Rikova, K. et al. (2007) Cell 131, 1190-203.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products