Product Pathways - Chromatin Regulation / Epigenetics
SMARCC2/BAF170 Antibody #8829
|8829S||100 µl (10 western blots)||---||In Stock||---|
|8829||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||162, 170||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting
Specificity / Sensitivity
SMARCC2/BAF170 Antibody recognizes endogenous levels of total SMARCC2/BAF170 protein (both isoforms 1 and 2).
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala223 of human SMARCC2/BAF170 protein. Antibodies are purified by protein A and peptide affinity chromatography.
ATP-dependent chromatin remodeling complexes play an essential role in the regulation of nuclear processes such as transcription and DNA replication and repair (1,2). The SWI/SNF chromatin remodeling complex consists of more than 10 subunits and contains a single molecule of either BRM or BRG1 as the ATPase catalytic subunit. The activity of the ATPase subunit disrupts histone-DNA contacts and changes the accessibility of crucial regulatory elements to the chromatin. The additional core and accessory subunits play a scaffolding role to maintain stability and provide surfaces for interaction with various transcription factors and chromatin (2-5). The interactions between SWI/SNF subunits and transcription factors, such as nuclear receptors, p53, Rb, BRCA1, and MyoD, facilitate recruitment of the complex to target genes for regulation of gene activation, cell growth, cell cycle, and differentiation processes (1,6-9).
SMARCC2/BAF170 is one of the core subunits of the SWI/SNF complex, which is necessary for efficient nucleosome remodeling by Brg1 in vitro (10). While SMARCC2/BAF170 has been shown to be part of the SWI/SNF complex in non-pluripotent cells, it is absent in pluripotent embryonic stem (ES) cells. Expression of SMARCC2/BAF170 has been shown to be up-regulated in neurons/neuronal progenitors upon differentiation of mouse ES cells with retinoic acid, and exogenous expression of SMARCC2/BAF170 leads to loss of stem cell pluripotency and self renewal (11).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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