Cell Signaling Technology

Product Pathways - Metabolism

Catalase Antibody #8841

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Endogenous 60 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Catalase Antibody recognizes endogenous levels of total catalase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile165 of human catalase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Catalase Antibody.

Background

Catalase catalyzes the conversion of hydrogen peroxide to water and oxygen (1). Studies show that overexpression of this antioxidant enzyme increases the ability of pancreatic β-cells to scavenge reactive oxygen species (ROS), thereby protecting pancreatic β-cells from oxidative stress (2). The pancreatic β-cells overexpressing this enzyme are also protected from hydrogen peroxide-mediated lipotoxicity, futher implying the importance of catalase in the pathogenesis of diabetes (3).

  1. Chelikani, P. et al. (2004) Cell Mol Life Sci 61, 192-208.
  2. Chen, H. et al. (2005) Diabetes 54, 1437-46.
  3. Elsner, M. et al. (2011) Diabetes 60, 200-8.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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