Product Pathways - Vesicle Trafficking
Eps15 Antibody #8855
|W IP||H M R Mk (Hm) (C) (B) (Pg) (Hr)||Endogenous||138-140||Rabbit|
Reactivity Key: H=Human M=Mouse R=Rat Hm=Hamster Mk=Monkey C=Chicken B=Bovine Pg=Pig Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Eps15 Antibody recognizes endogenous levels of total Eps15 protein. Based on sequence alignment, this antibody is predicted to cross-react with both Eps15a and Eps15b. This antibody does not cross-react with Eps15R.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Eps15 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Eps15 (EGFR pathway substrate 15) was originally discovered as a substrate for the kinase activity of EGFR (1). Eps15 has a tripartite structure comprising an amino terminal portion, which contains three evolutionarily conserved EH protein-protein interaction domains, a central putative coiled-coil region required for constitutive oligmerization, and a carboxy terminal domain containing multiple copies of the amino acid triplet Asp-Pro-Phe that constitute the AP2 binding domain. The carboxy terminal domain also contains two ubiquitin interaction motifs (UIMs), the last of which is indespensible for Eps15 binding to ubiquitin (1). Several lines of evidence support a role for Eps15 in clathrin-mediated endocytosis, including the endocytosis of synaptic vesicles. Eps15 binds to AP2 as well as other proteins involved in endocytosis and/or synaptic vesicle recycling, such as synaptojanin1 and epsin. Furthermore, Eps15 colocalizes with markers of the plasma membrane clathrin-coated pits and vesicles (2). Eps15 regulates the endosomal trafficking of c-Met (3) and EGFR (4), possibly by recruiting the ubiquitinated receptors to the rims of clathrin-coated pits through interaction between the ubiquitin tag and its UIMs.The EPS15 gene yields two isoforms that are believed to reside in distinct subcellular locations and are thus implicated in different facets of endosomal trafficking (5). Human EPS15 has been mapped to chromosome 1p31-p32, a region displaying several nonrandom chromosomal abnormalities, including deletions in neuroblastoma and translocations in acute lymphoblastic and myeloid leukemias. Research has shown two translocations t(1;11)(p32;q11) are found in rare cases of myeloid leukemia where the Eps15 gene was fused to the HRX gene, resulting in two reciprocal fusion genes (6).
- Fazioli, F. et al. (1993) Mol Cell Biol 13, 5814-28.
- Tebar, F. et al. (1996) J Biol Chem 271, 28727-30.
- Parachoniak, C.A. and Park, M. (2009) J Biol Chem 284, 8382-94.
- Torrisi, M.R. et al. (1999) Mol Biol Cell 10, 417-34.
- Roxrud, I. et al. (2008) J Cell Biol 180, 1205-18.
- Bernard, O.A. et al. (1994) Oncogene 9, 1039-45.
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For Research Use Only. Not For Use In Diagnostic Procedures.