Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Cytoskeletal Signaling

Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #8856

Applications Reactivity Sensitivity Isotype
IF-IC F H M R (Mk) (Dg) Endogenous Rabbit IgG

Applications Key:  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  Dg=Dog
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of keratin 17 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human keratin 17 protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A549 (blue) and HeLa (green) cells using Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (keratin 17 positive; left), HCC827 (keratin 17 negative, keratin 19 positive; middle), or SK-MEL-28 (keratin 17/19 negative; right) cells using Keratin 17 (D73C7) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) (blue pseudocolor). Actin filaments were labeled with Alexa Fluor® 488 phalloidin (green). Red = Propidium Iodide (PI)/RNase Staining Solution #4087 (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Keratin 17 (D73C7) XP® Rabbit mAb #4543.

Background

Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments (1,2). Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them useful as biomarkers (1). Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases (3-6).

  1. Moll, R. et al. (1982) Cell 31, 11-24.
  2. Chang, L. and Goldman, R.D. (2004) Nat Rev Mol Cell Biol 5, 601-13.
  3. Ramaekers, F.C. and Bosman, F.T. (2004) J Pathol 204, 351-4.
  4. Lane, E.B. and McLean, W.H. (2004) J Pathol 204, 355-66.
  5. Zatloukal, K. et al. (2004) J Pathol 204, 367-76.
  6. Owens, D.W. and Lane, E.B. (2004) J Pathol 204, 377-85.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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