Cell Signaling Technology

Product Pathways - Metabolism

Glucose-6-Phosphate Dehydrogenase Antibody #8866

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 58 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Glucose-6-Phosphate Dehydrogenase Antibody recognizes endogenous levels of total glucose-6-phosphate dehydrogenase protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human glucose-6-phosphate dehydrogenase protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Glucose-6-Phosphate Dehydrogenase Antibody.

Background

Glucose-6-phosphate dehydrogenase (G6PD) catalyses the first and rate-limiting step of the pentose phosphate pathway (1). The NADPH generated from this reaction is essential to protect cells from oxidative stress (1). Recent studies have shown that p53 interacts with G6PD and inhibits its activity, therefore suppressing glucose consumption through the pentose phosphate pathway (2). In cancer cells with p53 mutations, the increased glucose consumption is directed towards increased biosynthesis, which is critical for cancer cell proliferation (2).

  1. Au, S.W. et al. (2000) Structure 8, 293-303.
  2. Jiang, P. et al. (2011) Nat Cell Biol 13, 310-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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