Product Pathways - Cell Cycle / Checkpoint
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) #8880
|IF-IC F||H M R Mk||Endogenous||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
* Product-specific protocol.
Specificity / Sensitivity
Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate) recognizes endogenous levels of Rb protein only when phosphorylated at Ser807, Ser811, or at both sites. This antibody does not cross-react with Rb phosphorylated at Ser608.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser807/811 of human Rb protein.
Flow cytometic analysis of BT-549 (blue) and Jurkat (green) cells using Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb (Alexa Fluor® 594 Conjugate).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 549 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Phospho-Rb (Ser807/811) (D20B12) XP® Rabbit mAb #8516.
The retinoblastoma tumor suppressor protein, Rb, regulates cell proliferation by controlling progression through the restriction point within the G1-phase of the cell cycle (1). Rb has three functionally distinct binding domains and interacts with critical regulatory proteins including the E2F family of transcription factors, c-Abl tyrosine kinase, and proteins with a conserved LXCXE motif (2-4). Cell cycle-dependent phosphorylation by a CDK inhibits Rb target binding and allows cell cycle progression (5). Rb inactivation and subsequent cell cycle progression likely requires an initial phosphorylation by cyclin D-CDK4/6 followed by cyclin E-CDK2 phosphorylation (6). Specificity of different CDK/cyclin complexes has been observed in vitro (6-8) and cyclin D1 is required for Ser780 phosphorylation in vivo (9).
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- Geng, Y. et al. (2001) Proc Natl Acad Sci USA 98, 194-9.
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For Research Use Only. Not For Use In Diagnostic Procedures.