Cell Signaling Technology

Product Pathways - Transcription Factors

NRF2 (D1C9) Rabbit mAb #8882

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M Endogenous 98 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NRF2 (D1C9) Rabbit mAb recognizes endogenous levels of total mouse NRF2 protein. It also detects overexpressed human NRF2 protein. This antibody cross-reacts with a nonspecific band at 70 kDa.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Glu311 of human NRF2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from MEF cells, untreated (-) or MG132-treated (10 µM, 10 hr) (+), using NRF2 (D1C9) Rabbit mAb.

Background

The nuclear factor-like 2 (NRF2) transcriptional activator binds antioxidant response elements (ARE) of target gene promoter regions to regulate expression of oxidative stress response genes. Under basal conditions, the NRF2 inhibitor INrf2 (also called KEAP1) binds and retains NRF2 in the cytoplasm where it can be targeted for ubiquitin-mediated degradation (1). Small amounts of constitutive nuclear NRF2 maintains cellular homeostasis through regulation of basal expression of antioxidant response genes. Following oxidative or electrophilic stress, KEAP1 releases NRF2, thereby allowing the activator to translocate to the nucleus and bind to ARE-containing genes (2). The coordinated action of NRF2 and other transcription factors mediates the response to oxidative stress (3). Altered expression of NRF2 is associated with chronic obstructive pulmonary disease (COPD) (4). NRF2 activity in lung cancer cell lines directly correlates with cell proliferation rates, and inhibition of NRF2 expression by siRNA enhances anti-cancer drug-induced apoptosis (5).

  1. Cullinan, S.B. et al. (2004) Mol Cell Biol 24, 8477-86.
  2. Nguyen, T. et al. (2005) J Biol Chem 280, 32485-92.
  3. Jaiswal, A.K. (2004) Free Radic Biol Med 36, 1199-207.
  4. Suzuki, M. et al. (2008) Am J Respir Cell Mol Biol 39, 673-82.
  5. Homma, S. et al. (2009) Clin Cancer Res 15, 3423-32.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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