Secondary Antibodies
Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate) #8885
Protocols
- 8885:
- Flow
Specificity / Sensitivity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue), using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 detected with Anti-rabbit IgG (H+L), F(ab')2 Fragment (PE Conjugate).
Description
Anti-rabbit IgG (H+L), F(ab')2 Fragment was conjugated to phycoerythrin (PE) under optimal conditions and formulated at 1 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Directions for Use
The optimal dilution of the secondary antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most flow cytometry applications.
Applications
This product has been optimized for use as a secondary antibody in FLOW ctytometry.
Background
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
Application References
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The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
