Secondary Antibodies
Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) #8890
Protocols
- 8890:
- Flow, Immunofluorescence
Specificity / Sensitivity
F(ab')2 fragments are prepared from goat antibodies that have been adsorbed against pooled human serum, mouse serum, plasmacytoma/hybridoma proteins, and purified human paraproteins.
Flow Cytometry
Flow cytometric analysis of untreated Jurkat cells using Akt (5G3) Mouse mAb #2966 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (blue) compared to concentration-matched Mouse (G3A1) mAb IgG1 Isotype Control #5415 (red).
IF-IC
Immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677 detected with Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 594 Conjugate) (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
Anti-mouse IgG (H+L), F(ab')2 Fragment was conjugated to Alexa Fluor® 594 fluorescent dye under optimal conditions and formulated at 2 mg/ml. This F(ab')2 fragment results in less non-specific binding to cells through Fc receptors.
Directions for Use
The optimal dilution of the anti-species antibody should be determined for each application. However, a final dilution of 1:500 should yield acceptable results for most immunofluorescent applications.
Applications
This product has been optimized for use as a secondary antibody in immunofluorescent applications.
Background
Fluorescent anti-species IgG conjugates are ideal for flow cytometry and immunofluorescence. Cell Signaling Technology’s strict quality control procedures assure that each conjugate provides optimal specificity and fluorescence.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
