Cell Signaling Technology

Product Pathways - Growth Factors/Cytokines

Human Interleukin-17F (hIL-17F) #8906

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Source

Recombinant human IL-17F (hIL-17F) Arg31-Gln163 (Accession #NP_443104) was expressed in human 293 cells at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-17F contains no "tags" and the nonglycosylated protein has a calculated MW of 14,903. DTT-reduced protein migrates as a 22 kDa polypeptide. Lower mobility in SDS-PAGE is due to glycosylation. The non-reduced cystine-linked homodimer migrates as a 38 kDa protein. The expected amino-terminal RKIPK of recombinant hIL-17F was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-17F. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-17F was determined by its ability to induce mouse IL-6 production by 3T3 MEFs WT. The ED50 of each lot is between 30-80 ng/ml.

Coomassie Gel

Coomassie Gel

The purity of recombinant hIL-17F was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-17F and staining overnight with Coomassie Blue.

Bioactivity

Bioactivity

The production of mouse IL-6 by 3T3 MEFs WT cultured with increasing concentrations of hIL-17F was assessed. Media from cells incubated with hIL-17F for 24 hours was collected and assayed for mouse IL-6 by ELISA and the OD450-OD650 was determined.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-17F.

Formulation

With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-17F. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.

Background

IL-17F is a cystine-linked homodimeric pro-inflammatory cytokine produced by TH17 cells, a distinct CD4+ T cell subset (1). IL-17F induces the production of pro-inflammatory cytokines, antimicrobial peptides, and neutrophil chemoattractants such as IL-6, β-defensin, IL-8, CXCL1, and CXCL6 (1,2). As a result, IL-17F may provide an important link between adaptive and the innate immunity. IL-17F binds with high affinity to IL-17RC, expressed primarily in non-hematopoietic tissues, and with lesser affinity to IL-17RA, which is expressed in hematopoietic tissues (3). IL-17F binding activates the ERK1/2 MAP kinase and NF-κB pathways (1,4). IL-17F appears to be involved in mucosal immunity against bacterial infections and possibly some autoimmune conditions (1,5,6).

  1. Kolls, J.K. and Lindén, A. (2004) Immunity 21, 467-76.
  2. Liang, S.C. et al. (2006) J Exp Med 203, 2271-9.
  3. Kuestner, R.E. et al. (2007) J Immunol 179, 5462-73.
  4. Kawaguchi, M. et al. (2002) J Biol Chem 277, 15229-32.
  5. Zrioual, S. et al. (2009) J Immunol 182, 3112-20.
  6. Ishigame, H. et al. (2009) Immunity 30, 108-19.

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For Research Use Only. Not For Use In Diagnostic Procedures.

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