Product Pathways - Growth Factors/Cytokines
Human Leukemia Inhibitory Factor (hLIF) #8911
|8911LC||50 µg (With Carrier)||---||In Stock||---|
|8911LF||50 µg (Carrier Free)||---||In Stock||---|
|8911SF||10 µg (Carrier Free)||---||In Stock||---|
|8911SC||10 µg (With Carrier)||---||In Stock||---|
Already purchased this product? Write a Review.
Recombinant human LIF (hLIF) Ser23-Phe202 (Accession #P15018) was produced in E. coli at Cell Signaling Technology.
Based on amino acid sequencing, greater than 30% of recombinant hLIF has a Met on the amino-terminal Ser23 (MSPLP) and has a calculated MW of 19,847. The remainder starts at Ser23 (SPLPI), Pro24 (PLPIT), or Pro26 (PITPV). DTT-reduced protein migrates as a 20 kDa polypeptide and non-reduced protein migrates as a 17 kDa polypeptide due to intramolecular cystines.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hLIF. All lots are greater than 98% pure.
The bioactivity of recombinant hLIF was determined in a TF-1 cell proliferation assay. The ED50 of each lot is between 0.2-1.5 ng/ml.
The purity of recombinant hLIF was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hLIF and staining overnight with Coomassie Blue.
The proliferation of TF-1 cells treated with increasing concentrations of hLIF was assessed. After 72 hr treatment with hLIF, cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
Western blot analysis of extracts from TF-1 cells untreated or treated with hLIF for 10 min, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).
Less than 0.01 ng endotoxin/1 μg hLIF.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hLIF. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.