Product Pathways - Growth Factors/Cytokines
Human Insulin-like Growth Factor I (hIGF-I) #8917
|8917LF||250 µg (Carrier Free)||---||In Stock||---|
|8917LC||250 µg (With Carrier)||---||In Stock||---|
|8917SC||50 µg (With Carrier)||---||In Stock||---|
|8917SF||50 µg (Carrier Free)||---||In Stock||---|
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Recombinant human IGF-I (hIGF-I) Gly49-Ala118 (Accession #P01343) was produced in E. coli at Cell Signaling Technology.
Recombinant hIGF-I has a Met on the amino terminus and has a calculated MW of 7,785. DTT-reduced and non-reduced protein migrate as 6 kDa polypeptides. The expected amino-terminal MGPET of recombinant hIGF-I was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIGF-I. All lots are greater than 98% pure.
The bioactivity of recombinant hIGF-I was determined in a cell proliferation assay using primary human dermal fibroblasts. The ED50 of each lot is between 2-8 ng/ml.
The purity of recombinant hIGF-I was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIGF-I and staining overnight with Coomassie Blue.
The proliferation of primary human dermal fibroblasts treated with increasing concentrations of hIGF-I was assessed. After 72-hour treatment with hIGF-I cells were incubated with a tetrazolium salt and the OD450 - OD650 was determined.
Western blot analysis of extracts from human dermal fibroblasts untreated or treated with hIGF-I for 10 minutes, using Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb #4060 (upper) and Akt1 (C73H10) Rabbit mAb #2938 (lower).
Less than 0.01 ng endotoxin/1 μg hIGF-I.
With carrier: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl and 20 μg BSA per 1 μg hIGF-I. Carrier free: Lyophilized from a 0.22 μm filtered solution of 20 mM citrate, pH 3.0 containing 100 mM NaCl.
Most circulating endocrine acting IGF-I is produced by hepatocytes, and paracrine or autocrine acting IGF-I is produced by defined cell types within specific tissues (1,2). Many neoplastic cells produce IGF-I, which regulates a number of cellular processes including energy metabolism, proliferation, and cell survival (3,4). IGF-I activity is regulated by one or more of the six extracellular IGF-binding proteins (IGFBPs). IGFBPs bind to IGF-I and most inhibit IGF-I binding to IGF-I receptor (IGFIR) (1,2). Some IGFBPs may increase cell responses to IGF-I. Binding of IGF-I to IGFIR activates the Akt, JNK, and Erk pathways (2). IGF-I and IGFIR are frequently expressed by cancer cells and may contribute to the proliferation and viability of a number of cancer types (1,2).
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- 3021 Phospho-IGF-I Receptor β (Tyr1131)/Insulin Receptor β (Tyr1146) Antibody
- 3027 IGF-I Receptor β Antibody
- 3018 IGF-I Receptor β (111A9) Rabbit mAb
- 3922 IGFBP2 Antibody
- 4568 Phospho-IGF-I Receptor β (Tyr980) (C14A11) Rabbit mAb
- 4060 Phospho-Akt (Ser473) (D9E) XP® Rabbit mAb
- 2938 Akt1 (C73H10) Rabbit mAb
For Research Use Only. Not For Use In Diagnostic Procedures.
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