Product Pathways - Growth Factors/Cytokines

• pathway
• application references
• companion products
• datasheet PDF
• MSDS PDF
• protocols

Human Interleukin-8 (hIL-8) #8921

Request pricing for multi-milligram amounts.

Source

Recombinant human IL-8 (hIL-8) Ser28-Ser99 (Accession #NM_000584) was produced in E.coli at Cell Signaling Technology.

Molecular Characterization

Recombinant hIL-8 does not have a Met on the amino terminus and has a calculated MW of 8,386. DTT-reduced and non-reduced protein migrate as 8 kDa polypeptides. The expected amino-terminal SAKEL of recombinant hIL-8 was verified by amino acid sequencing.

Purity

>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-8. All lots are greater than 98% pure.

Bioactivity

The bioactivity of recombinant hIL-8 was determined by upregulation of CD11b expression on primary human neutrophils. The ED₅₀ of each lot is between 1-5 ng/ml.

Endotoxin

Less than 0.01 ng endotoxin/1 μg hIL-8.

Formulation

With carrier:SC: A 0.22 μm filtered solution of 0.25 mg/ml hIL-8 in PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-8.LC: A 0.22 μm filtered solution of 0.33 mg/ml hIL-8 in PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-8.Carrier free:SF: A 0.22 μm filtered solution of 0.25 mg/ml hIL-8 in PBS, pH 7.2.LF: A 0.22 μm filtered solution of 0.33 mg/ml hIL-8 in PBS, pH 7.2.

Background

The prototypical CXC chemokine, IL-8, is best known for effects on neutrophils, specifically its ability to act as a chemoattractant and activate degranulation and respiratory burst (1,2,3).IL-8 is produced by a number of cell types including monocytes, T cells, neutrophils, fibroblasts, endothelial cells, and others (1). In addition to its effects on neutrophils, IL-8 promotes angiogenesis, inhibits endothelial cell apoptosis, and promotes the proliferation of melanoma cells in an autocrine fashion (1). As a result, IL-8 may be a contributing factor to the development of certain cancers (1,2). There are two distinct receptors for IL-8, CXCR1 and CXCR2; both are G protein-coupled receptors (1). Ligand binding induces Ca²⁺ mobilization and activates the PI3K, PKC, Rho, and Rac pathways (1,3).

1. Payne, A.S. and Cornelius, L.A. (2002) J Invest Dermatol 118, 915-22.
2. Brat, D.J. et al. (2005) Neuro Oncol 7, 122-33.
3. Mukaida, N. (2003) Am J Physiol Lung Cell Mol Physiol 284, L566-77.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9872 Phosphate Buffered Saline (PBS-1X) pH7.2 (Sterile)

---

For Research Use Only. Not For Use In Diagnostic Procedures.