Product Pathways - Growth Factors/Cytokines
Human Interleukin-22 (hIL-22) #8931
|8931LC||50 µg (With Carrier)||---||In Stock||---|
|8931LF||50 µg (Carrier Free)||---||In Stock||---|
|8931SC||10 µg (With Carrier)||---||In Stock||---|
|8931SF||10 µg (Carrier Free)||---||In Stock||---|
Already purchased this product? Write a Review.
Recombinant human IL-22 (hIL-22) Ala34-Ile179 (Accession #NP_065386) was expressed in human 293 cells at Cell Signaling Technology.
Recombinant hIL-22 contains no "tags" and the nonglycosylated protein has a calculated MW of 16,749. DTT-reduced and non-reduced protein migrate as 34 kDa polypeptides. Lower mobility and heterogeneity in SDS PAGE are due to glycosylation. The expected amino-terminal APISS of recombinant hIL-22 was verified by amino acid sequencing.
>98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hIL-22. All lots are greater than 98% pure.
The bioactivity of recombinant IL-22 was determined by its ability to induce IL-10 production by COLO 205 cells. The ED50 of each lot is between 100-300 pg/ml.
The purity of recombinant hIL-22 was determined by SDS-PAGE of 6 µg reduced (+) and non-reduced (-) recombinant hIL-22 and staining overnight with Coomassie Blue.
The production of IL-10 by COLO 205 cells cultured with increasing concentrations of hIL-22 was assessed. Media from the cells incubated with hIL-22 for 48 hours was collected and assayed for IL-10 by ELISA and the OD450-OD650 was determined.
Western blot analysis of extracts from COLO 205 cells, untreated or treated with hIL-22 for 15 minutes, using Phospho-Stat3 (Tyr705) (D3A7) XP® Rabbit mAb #9145 (upper) or Stat3 Antibody #9132 (lower).
Less than 0.01 ng endotoxin/1μg hIL-22.
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hIL-22. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
IL-22, a member of the IL-10 family (1,2), is expressed by Th17 CD4+ T cells, activated T cells, Th1 cells and NK cells (3). IL-22 induces proinflammatory responses, drives production of antimicrobial peptides, and is involved in tissue-repair and wound-healing responses (1). The IL-22 receptor is a heterodimer of IL-22R1 and IL-10R2 (4). Expression of IL-22R is restricted to tissue-resident cells, particularly those of epithelial origin, whereas the IL-10R2 chain is expressed in many more cell types. IL-22 induces phosphorylation of Jak1 and Tyk2, leading to activation of Stat3 and, to a lesser extent, Stat1 and Stat5 (1). IL-22 responses can involve activation of the MEK-ERK-RSK, JNK-SAPK, and p38 pathways (1). Elevated levels of IL-22 have been associated with Crohn's disease and rheumatoid arthritis. IL-22 plays an essential role in host response to the pulmonary pathogen Klebsiella pneumoniae (5).
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.