Cell Signaling Technology

Product Pathways - Neuroscience

GAP43 (D9C8) Rabbit mAb #8945

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F H M R Endogenous 38, 43 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

GAP43 (D9C8) Rabbit mAb recognizes endogenous levels of total GAP43 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys30 of human GAP43 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse neonatal brain and rat fetal brain using GAP43 (D9C8) Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of rat hippocampus (left) and retina (right) using GAP43 (D9C8) Rabbit mAb (green) and GFAP (GA5) Mouse mAb (Alexa Fluor® 555 Conjugate) #3656 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

GAP43 is a nervous system specific, growth-associated protein enriched in growth cones and areas of high plasticity (1). Phosphorylation of GAP43 at Ser41 by PKC is regulated by intracellular Ca2+ and affects the ability of GAP43 to bind calmodulin (2,3). GAP43 is integral to growth cone formation, neurite outgrowth, and the development of a functional cerebral cortex (4,5). Aberrant GAP43 expression can be seen in patients diagnosed with schizophrenia and Alzheimer's disease (6,7).

  1. Biewenga, J.E. et al. (1996) Acta Biochim Pol 43, 327-38.
  2. Dekker, L.V. and Parker, P.J. (1997) J Biol Chem 272, 12747-53.
  3. Chapman, E.R. et al. (1991) J Biol Chem 266, 207-13.
  4. Aigner, L. and Caroni, P. (1993) J Cell Biol 123, 417-29.
  5. Shen, Y. et al. (2008) Cerebellum 7, 451-66.
  6. Sower, A.C. et al. (1995) Mol Chem Neuropathol 24, 1-11.
  7. de la Monte, S.M. et al. (1995) Am J Pathol 147, 934-46.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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