Product Pathways - Screening Technologies
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb #8954
|8954S||100 µl (20 western blots)||---||In Stock||---|
|8954||carrier free and custom formulation / quantity||email request|
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|W||1:2000||All Species Expected||Endogenous||N/A||Rabbit IgG|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Specificity / Sensitivity
Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb recognizes a broad range of tyrosine-phosphorylated proteins and peptides. This antibody does not cross-react with proteins or peptides containing phospho-Ser or phospho-Thr residues.
Source / Purification
Monoclonal antibody is produced by immunizing animals with phospho-tyrosine containing peptides.
Western blot analysis of extracts from A-431 cells, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb. Western blot image was obtained using the Odyssey® Infrared Imaging System (LI-COR® Biotechnology).
Immunoprecipitation of phospho-tyrosine proteins from A-431 cell extracts, untreated (-) or treated with Human Epidermal Growth Factor (hEGF) #8916 (100 ng/ml, 5 min; +) (lanes 3 and 4), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb. Western blot analysis was performed using the same antibody. Lanes 1 and 2 are 10% input.
Flow cytometric analysis of K-562 cells, untreated (green) or Gleevec®-treated (blue), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb.
Confocal immunofluorescent analysis of C2C12 cells, serum-starved (left), treated with H2O2 (2 mM, 10 min; middle), or treated with H2O2 followed by λ phosphatase (right), using Phospho-Tyrosine (P-Tyr-1000) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Tyrosine phosphorylation plays a key role in cellular signaling (1). Research studies have shown that in cancer, unregulated tyrosine kinase activity can drive malignancy and tumor formation by generating inappropriate proliferation and survival signals (2). Antibodies specific for phospho-tyrosine (3,4) have been invaluable reagents in these studies. The phospho-tyrosine monoclonal antibodies developed by Cell Signaling Technology are exceptionally sensitive tools for studying tyrosine phosphorylation and monitoring tyrosine kinase activity in high throughput drug discovery.
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
LI-COR® is a registered trademark of LI-COR, Inc.
Odyssey® is a registered trademark of LI-COR, Inc.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
Use of Cell Signaling Technology (CST) Motif Antibodies within certain methods (e.g., U.S. Patents No. 7,198,896 and 7,300,753) may require a license from CST. For information regarding academic licensing terms please have your technology transfer office contact CST Legal Department at CST_ip@cellsignal.com. For information regarding commercial licensing terms please contact CST Pharma Services Department at firstname.lastname@example.org.