Cell Signaling Technology

Product Pathways - Metabolism

PLA2G1B Antibody #8962

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 16 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PLA2G1B Antibody recognizes endogenous levels of total PLA2G1B protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala131 of human PLA2G1B protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from human pancreas and kidney tissue using PLA2G1B Antibody (upper) and β-Actin (13E5) Rabbit mAb #4970 (lower).

Background

Phospholipase A2 (PLA2) is a superfamily of enzymes that hydrolyze glycero-3-phosphocholines and release fatty acids and lysophospholipids (1). PLA2G1B is a member of this superfamily in the 1B group that is expressed most highly in the pancreatic acinar cells (2). Evidence suggests that PLA2G1B plays a role in the absorption and storage of extra energy as fats are metabolized (1,2). Lysophospholipids generated by PLA2G1B inhibit fatty acid oxidation in the liver and reduce energy expenditure, leading to diet-induced obesity and type 2 diabetes with a high fat diet (1). Therefore, a potential intervention of obesity and diabetes could target PLA2G1B in the digestive tract (2).

  1. Labonté, E.D. et al. (2010) FASEB J 24, 2516-24.
  2. Cash, J.G. et al. (2010) Int J Obes (Lond), Epub ahead of print.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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