Product Pathways - Cell Cycle / Checkpoint
NuMA (D49H4) Rabbit mAb #8967
PhosphoSitePlus® protein, site, and accession data: NuMA-1
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IF-IC | H Mk | Endogenous | 238 | Rabbit IgG |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
NuMA (D49H4) Rabbit mAb detects endogenous levels of total NuMA protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human NuMA protein.
Western Blotting
Western blot analysis of extracts from various cell lines using NuMA (D49H4) Rabbit mAb.
IF-IC
Confocal immunofluorescent analysis of MCF7 cells using NuMA (D49H4) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Background
The nuclear mitotic apparatus protein (NuMA) is a coiled coil protein involved in the formation and maintenance of the mitotic spindle. NuMA plays a role in chromatin organization during interphase, which influences mammary epithelial differentiation (1,2). During apoptosis, carboxy-terminal cleavage of NuMA may amplify signaling in the cell death pathway (2). NuMA is phosphorylated at numerous sites, with phosphorylation at Ser395 occurring in an ATM/ATR-dependent manner in response to DNA damage (3,4).
- Abad, P.C. et al. (2007) Mol Biol Cell 18, 348-61.
- Lin, H.H. et al. (2007) J Biomed Sci 14, 681-94.
- Stokes, M.P. et al. (2007) Proc Natl Acad Sci USA 104, 19855-60.
- Matsuoka, S. et al. (2007) Science 316, 1160-6.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 3888 NuMA Antibody
- 3429 Phospho-NuMA (Ser395) Antibody
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
