Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

CLIP1/CLIP170 Antibody #8977

Applications Reactivity Sensitivity MW (kDa) Source
W H M Mk Endogenous 170 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CLIP1/CLIP170 Antibody recognizes endogenous levels of total CLIP1/CLIP170 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CLIP1/CLIP170 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using CLIP1/CLIP170 Antibody.

Background

Microtubules (MTs) are polarized filaments composed of α/β tubulin heterodimers. The slower growing (minus) ends of MTs are located at centrosomes, and the faster growing (plus) ends extend into the cell periphery. Regulation of MT dynamics is important for multiple cellular functions, including cell division, migration, adhesion, membrane trafficking, and polarity (reviewed in 1).Cytoplasmic linker protein 1/170 (CLIP1/CLIP170) localizes to the plus ends of MTs (2), and binds to the Rac1/cdc42 effector protein IQGAP1. This complex is involved in establishing cell polarity (3).CLIP1/CLIP170 also facilitates MT-dependent organelle transport (4), and phosphorylation of CLIP1/CLIP170 by PLK1 and CK2 is required for efficient microtubule–kinetochore attachments in mitosis (5).

  1. Wiese, C. and Zheng, Y. (2006) J Cell Sci 119, 4143-53.
  2. Perez, F. et al. (1999) Cell 96, 517-27.
  3. Fukata, M. et al. (2002) Cell 109, 873-85.
  4. Lomakin, A.J. et al. (2011) Mol Biol Cell 22, 4029-37.
  5. Li, H. et al. (2010) EMBO J 29, 2953-65.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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