Product Pathways - Translational Control
DDX6/RCK (D26C11) Rabbit mAb #8988
|W||H M R Mk (X)||Endogenous||54||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey X=Xenopus
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
DDX6/RCK (D26C11) Rabbit mAb recognizes endogenous levels of total DDX6/RCK protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala130 of human DDX6/RCK protein.
DDX6, also known as RCK and p54, was identified as a proto-oncogene product and is a member of the ATP-dependent DEAD box helicase family (1,2). This protein interacts with translation initiation factor eIF4E in the cytoplasmic P-bodies (3) and represses mRNA translation (4). DDX6 is a component of the miRNA induced silencing complex (miRISC) and interacts with Argonaute 1 (Ago1) and Argonaute 2 (Ago2) proteins in vitro and in vivo (5), functioning in miRNA-mediated translational repression (5). Depletion of DDX6 leads to the disruption of cytoplasmic P-bodies indicating that it is required for P-body formation (5).
- Akao, Y. et al. (1995) Cancer Res 55, 3444-9.
- Weston, A. and Sommerville, J. (2006) Nucleic Acids Res 34, 3082-94.
- Andrei, M.A. et al. (2005) RNA 11, 717-27.
- Minshall, N. and Standart, N. (2004) Nucleic Acids Res 32, 1325-34.
- Chu, C.Y. and Rana, T.M. (2006) PLoS Biol 4, e210.
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For Research Use Only. Not For Use In Diagnostic Procedures.