Cell Signaling Technology

Product Pathways - NF-kB Signaling

IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 647 Conjugate) #8993

Applications Reactivity Sensitivity Isotype
F H M R Mk B Pg GP Endogenous Mouse IgG1

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  B=Bovine  Pg=Pig  GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

* Product-specific protocol.

Specificity / Sensitivity

IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total IκBα protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a GST-IκBα fusion protein corresponding the amino terminus of human IκBα protein.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of HeLa cells using IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) (Alexa Fluor® 647 Conjugate) (green) compared to a nonspecific negative control antibody (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated IκBα (L35A5) Mouse mAb (Amino-terminal Antigen) #4814.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

  1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
  2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
  3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
  4. Brown, K. et al. (1995) Science 267, 1485-8.
  5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
  6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
  7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
  8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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