Product Pathways - Chromatin Regulation / Epigenetics
ChIP-Grade Protein G Agarose Beads #9007
ChiP-Grade Protein G Agarose Beads are an affinity matrix for the small-scale isolation of immunocomplexes from chromatin immunoprecipitations (ChIP assays). A truncated form of recombinant Protein G is covalently coupled to agarose beads. Protein G exhibits high affinity for subclasses of IgG from many species (including human, rabbit, mouse, rat and sheep) and can be used for immunoprecipitation assays with these antibodies. The beads are stored in buffer containing BSA (500 µg/ml) and sonicated salmon sperm DNA (200 µg/ml) to block non-specific binding of proteins and DNA during isolation of immunocomplexes.
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and either Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620 (lane 2), Rpb1 CTD (4H8) Mouse mAb #2629 (lane 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (lane 4), or Normal Rabbit IgG #2729 (lane 5). Purified DNA was analyzed by standard PCR methods using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. PCR products were observed for each primer set in the input sample (lane 1) and various protein-specific immunoprecipitations, but not in the immunoprecipitation using Normal Rabbit IgG #2729 (lane 5).
Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The relative abundance of each DNA sequence enriched by protein-specific immunoprecipitations is compared to the amount of the same DNA sequence enriched by the non-specific Normal Rabbit IgG #2729 (background).
Directions for Use
Vortex tube briefly to resuspend the beads. Add 30 μl of bead slurry to each chromatin immunoprecipitation (ChIP) reaction. For bead washing and subsequent elution of immunocomplexes, the beads can be separated from solution by a brief 1 minute centrifugation in a microcentrifuge at 6,000 rpm. Resuspend the beads in solution by gently vortexing or rocking the tube.
Supplied in 10 mM Tris-HCl (pH 7.5), 1 mM EDTA, 500 µg/ml BSA, 200 µg/ml sonicated salmon sperm DNA and 0.05% sodium azide. Store at 4ºC.
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- 9002 SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads)
- 2729 Normal Rabbit IgG
- 9725 Di-Methyl-Histone H3 (Lys4) (C64G9) Rabbit mAb
- 9726 Di-Methyl-Histone H3 (Lys4) Antibody
- 9751 Tri-Methyl-Histone H3 (Lys4) (C42D8) Rabbit mAb
- 9753 Di-Methyl-Histone H3 (Lys9) Antibody
- 4069 Pan-Methyl-Histone H3 (Lys9) Antibody
- 9756 Tri-Methyl-Histone H3 (Lys27) Antibody
- 9649 Acetyl-Histone H3 (Lys9) (C5B11) Rabbit mAb
- 9677 Acetyl-Histone H3 (Lys9/Lys14) Antibody
- 2629 Rpb1 CTD (4H8) Mouse mAb
For Research Use Only. Not For Use In Diagnostic Procedures.