Product Pathways - Metabolism
Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate) #9016
PhosphoSitePlus® protein, site, and accession data: insulin
| Applications | Reactivity | Sensitivity | Isotype |
|---|---|---|---|
| IF-F F | H M R | Endogenous | Rabbit IgG |
Applications Key:
IF-F=Immunofluorescence (Frozen)
F=Flow Cytometry
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9016:
- Flow, Immunofluorescence
Specificity / Sensitivity
Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total insulin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human insulin.
Flow Cytometry
Flow cytometric analysis of HeLa (blue) and β-TC-6 (green) cells using Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate).
IF-F
Confocal immunofluorescent analysis of rat pancreas using Insulin (C27C9) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct immunofluorescent analysis in rat cells and flow cytometry in human and mouse cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Insulin (C27C9) Rabbit mAb #3014.
Background
The maintenance of glucose homeostasis is an essential physiological process that is regulated by hormones. An elevation in blood glucose levels during feeding stimulates insulin release from pancreatic β cells through a glucose sensing pathway (1). Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Insulin stimulates glucose uptake from blood into skeletal muscle and adipose tissue. Insulin deficiency leads to type 1 diabetes mellitus (2).
- Straub, S.G. and Sharp, G.W. (2002) Diabetes Metab. Res. Rev. 18, 451-463.
- Concannon, P. et al. (1998) Nat. Genet. 19, 292-296.
Application References
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Rabbit monoclonal antibody is produced under license (granting certain rights including those under U. S. Patent No. 5,675,063) from Epitomics, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
