Product Pathways - Neuroscience
Synaptophysin (7H12) Mouse mAb (IF Formulated) #9020
PhosphoSitePlus® protein, site, and accession data: SYP
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| IF-F | H M R | Endogenous | 38 | Mouse IgG1 |
Applications Key:
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9020:
- Immunofluorescence
Specificity / Sensitivity
Synaptophysin (7H12) Mouse mAb (IF Formulated) recognizes endogenous levels of total synaptophysin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to the carboxy terminus of human synaptophysin protein.
IF-F
Confocal immunofluorescent analysis of mouse brain (left) and mouse retina (right) using Synaptophysin (7H12) Mouse mAb (IF Formulated) (green) and CNPase (D83E10) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #5715 (red, left). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Background
Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).
- Wiedenmann, B. et al. (1986) Proc Natl Acad Sci USA 83, 3500-4.
- Valtorta, F. et al. (2004) Bioessays 26, 445-53.
- Arthur, C.P. and Stowell, M.H. (2007) Structure 15, 707-14.
- Bonanomi, D. et al. (2007) Biochem J 404, 525-34.
Application References
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- 4410 Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate)
- 9997 Tris Buffered Saline with Tween 20 (TBST-10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
For Research Use Only. Not For Use In Diagnostic Procedures.
