Cell Signaling Technology

Product Pathways - Adhesion

Integrin α2b Antibody #9028

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 137 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Integrin α2b Antibody recognizes endogenous levels of total integrin α2b protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human integrin α2b protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from KMOE-2 and TF-1 cells using Integrin α2b Antibody.

Background

Integrins are α/β heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 α and 8 β subunits that form 24 known integrins with distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with the extracellular environment (inside-out signaling) (1,2).

Integrin α2b is a cell-surface adhesion molecule that undergoes proteolytic cleavage to yield disulfide-linked heavy and light chains, which together with glycoprotein IIIb forms a major fibrinogen receptor in activated platelets (3). Researchers have shown a link between mutations in the ITGA2B and Glanzmann’s thrombasthenia (GT), a platelet function defect characterized by lack of platelet aggregation (4,5).

  1. Hood, J.D. and Cheresh, D.A. (2002) Nat Rev Cancer 2, 91-100.
  2. Liu, S. et al. (2000) J Cell Sci 113 ( Pt 20), 3563-71.
  3. Calvete, J.J. et al. (1990) FEBS Lett 272, 37-40.
  4. Vannier, C. et al. (2010) Klin Padiatr 222, 150-3.
  5. Nurden, A.T. et al. (2011) Blood 118, 5996-6005.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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