Product Pathways - Development
Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb #9030
|W IP||H M Mk||Endogenous||92||Rabbit IgG|
Reactivity Key: H=Human M=Mouse Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb recognizes endogenous levels of β-catenin protein only when acetylated at Lys49.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic acetylated peptide corresponding to residues surrounding Lys49 of human β-catenin protein.
Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing CBP (+), using Acetyl-β-Catenin (Lys49) (D7C2) Rabbit mAb (upper), β-Catenin (D10A8) XP® Rabbit mAb #8480 (middle), and β-Actin (D6A8) Rabbit mAb #8457 (lower).
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
Lys49 lies in a region that contains several Ser/Thr residues whose phosphorylation status regulates the stability of β-catenin. Research studies have shown that Lys49 is one of few residues frequently mutated in thyroid anaplastic carcinoma (9). CREB-binding protein (CBP) binds and acetylates β-catenin at Lys49 (10,11).
- Cadigan, K.M. and Nusse, R. (1997) Genes Dev. 11, 3286-3305.
- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
- Polakis, P. (2000) Genes Dev 14, 1837-51.
- Takemaru, K.I. and Moon, R.T. (2000) J Cell Biol 149, 249-54.
- Wolf, D. et al. (2002) J Biol Chem 277, 25562-7.
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For Research Use Only. Not For Use In Diagnostic Procedures.