Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

SPT5 Antibody #9033

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk (Hm) (B) (Dg) (GP) (Hr) Endogenous 150 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  B=Bovine  Dg=Dog  GP=Guinea Pig  Hr=Horse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SPT5 Antibody recognizes endogenous levels of total SPT5 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly803 of human SPT5 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using SPT5 Antibody.

Background

DRB-sensitivity inducing factor (DSIF), a heterodimer composed of SPT4 and SPT5, is capable of both facilitating and inhibiting RNA polymerase II (RNAPII) activity (1-3). DSIF, together with NELF (Negative Elongation Factor), inhibits RNAPII elongation, resulting in promoter proximal pausing of RNAPII as it awaits additional signaling to resume transcription (4). The release of promoter proximal pausing is signaled through phosphorylation of the RNAPII C-terminal domain (CTD) and NELF by positive transcription elongation factor (P-TEFb) (5). P-TEFb also phosphorylates SPT5 at Thr4 within the evolutionarily conserved heptapeptide repeat motif. This phosphorylation event switches DSIF from a transcriptional repressor to an activator where it becomes a critical factor for transcriptional elongation (6,7).

  1. Wada, T. et al. (1998) Genes Dev 12, 343-56.
  2. Saunders, A. et al. (2006) Nat Rev Mol Cell Biol 7, 557-67.
  3. Gomes, N.P. et al. (2006) Genes Dev 20, 601-12.
  4. Yamaguchi, Y. et al. (1999) Cell 97, 41-51.
  5. Buratowski, S. (2009) Mol Cell 36, 541-6.
  6. Wada, T. et al. (1998) EMBO J 17, 7395-403.
  7. Yamada, T. et al. (2006) Mol Cell 21, 227-37.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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