Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Ikaros (D10E5) Rabbit mAb #9034

Applications Reactivity Sensitivity MW (kDa) Isotype
W IF-IC F H Endogenous 50-70 Rabbit IgG

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Ikaros (D10E5) Rabbit mAb recognizes endogenous levels of total Ikaros protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg439 of human Ikaros protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Ikaros (D10E5) Rabbit mAb (upper) or β-actin (D6A8) Rabbit mAb #8457 (lower). Western blot results confirm absence of detectable Ikaros protein in HeLa cells, in accordance with the immunofluorescence analysis.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Ramos cells using Ikaros (D10E5) Rabbit mAb (blue) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of Jurkat cells (positive; left) and HeLa cells (negative; right) using Ikaros (D10E5) Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red).


Background

Ikaros family proteins, characterized by the presence of an N-terminal zinc finger DNA-binding domain and a C-terminal dimerization domain, belong to the Kruppel transcription factor superfamily. The Ikaros family includes Ikaros, Aiolos, Helios, and possibly EOS and Pegasus (1). They can form homodimers and heterodimers with other members of the Ikaros family. Due to differential splicing, multiple isoforms can be generated and some behave in a dominant negative manner upon dimerization (2).Ikaros, also known as Ikaros family zinc finger protein 1 (IKZF1) and Lymphoid transcription factor 1 (LYF-1), is expressed abundantly in blood cells. Genetic studies in mice demonstrate that Ikaros is important for the normal development of B, T, natural killer, and dendritic cells, and that it functions as a tumor suppressor (3,4). Research studies have shown that imbalanced expression of different Ikaros isoforms, as well as deletions of Ikaros are associated with hematologic malignancies in humans (5-7).

  1. Perdomo, J. et al. (2000) J Biol Chem 275, 38347-54.
  2. Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
  3. Georgopoulos, K. et al. (1997) Annu Rev Immunol 15, 155-76.
  4. Nichogiannopoulou, A. et al. (1998) Semin Immunol 10, 119-25.
  5. Tonnelle, C. et al. (2002) Leuk Lymphoma 43, 29-35.
  6. Rebollo, A. and Schmitt, C. (2003) Immunol Cell Biol 81, 171-5.
  7. Martinelli, G. et al. (2009) J Clin Oncol 27, 5202-7.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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