Cell Signaling Technology

Product Pathways - NF-kB Signaling

NDP52 Antibody #9036

Applications Reactivity Sensitivity MW (kDa) Source
W IP H Endogenous 52 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NDP52 Antibody recognizes endogenous levels of total NDP52 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro372 of human NDP52 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct encoding full-length human NDP52 (hNDP52; +), using NDP52 Antibody.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NDP52 Antibody.

Background

NDP52 (CALCOCO2) is ubiquitously expressed and composed of an amino-terminal SKICH domain, followed by a coiled-coil domain, and two zinc finger domains (1). It has recently been reported to act as an autophagy receptor that binds cytosolic ubiquitinated bacteria, leading to autophagy activation and pathogen clearance (1-3). NDP52 binds ubiquitin through its zinc finger domain and simultaneously binds LC3, which directs the bacteria into autophagosomes. In addition, NDP52 interacts with Nap1 and SINTBAD to recruit TBK1 to ubiquitinated bacteria (1).

  1. Thurston, T.L. et al. (2009) Nat Immunol 10, 1215-21.
  2. Cemma, M. et al. (2011) Autophagy 7, 341-5.
  3. Mostowy, S. et al. (2011) J Biol Chem 286, 26987-95.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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