Cell Signaling Technology

Product Pathways - Stem Cell and Lineage Markers

Fragilis Antibody #9037

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Endogenous 12 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Fragilis Antibody recognizes endogenous levels of total fragilis protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln16 of mouse fragilis protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NTERA-2 cl.D1, F9, and mouse iPS (miPS) cells using Fragilis Antibody.

Background

Fragilis (Ifitm3) belongs to the interferon (IFN)-inducible transmembrane protein family (1), which consists of five highly similar transmembrane proteins that are conserved among mammalian species (2). It is thought to be the first gene to mark the onset of germ cell competence during embryonic development in mice (3). Cells expressing high levels of fragilis protein go on to express stella, a protein found exclusively in lineage-restricted germ cells (3). Studies on fragilis family members could help elucidate the mechanisms through which interferons act as signaling molecules during embryonic development (2).

  1. Deblandre, G.A. et al. (1995) J Biol Chem 270, 23860-6.
  2. Lange, U.C. et al. (2003) BMC Dev Biol 3, 1.
  3. Saitou, M. et al. (2002) Nature 418, 293-300.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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