Cell Signaling Technology

Product Pathways - Development

NDRG4 (D4A6) Rabbit mAb #9039

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-F H M R (Mk) Endogenous 37-45 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

NDRG4 (D4A6) Rabbit mAb recognizes endogenous levels of total NDRG4 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly217 of human NDRG4 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from human cerebellum, mouse brain, and rat brain using NDRG4 (D4A6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using NDRG4 (D4A6) Rabbit mAb.

Western Blotting

Western Blotting

Western blot analysis of 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human NDRG4 (hNDRG4-Myc/DDK, +), using NDRG4 (D4A6) Rabbit mAb.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistichemical analysis of paraffin-embedded human lung carcinoma using NDRG4 (D4A6) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brain using NDRG4 (D4A6) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide(right).

IF-F

IF-F

Confocal immunofluorescent analysis of adult mouse brain using NDRG4 (D4A6) Rabbit mAb and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 555 Conjugate) #8039 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The NDRG (N-Myc downstream-regulated gene) family, consisting of NDRG1, NDRG2, NDRG3, and NDRG4, are a group of structurally related proteins with roles in cell proliferation, differentiation, apoptosis, stress responses, and cell migration/metastasis (1-3). NDRG1 was originally identified as a protein that was up-regulated in N-Myc knockout mice (1). Proteins in the NDRG family, particularly NDRG1 and NDRG2, have been reported to be down-regulated in various cancer tissues, and have been suggested to function as a tumor suppressors (4,5).

Unlike other members of the family that are widely expressed, NDRG4/Bdm1 expression is restricted primarily to the heart and brain (2,3,6,7). Multiple alternatively spliced isoforms of NDRG4 have been reported (3,7). Expression of NDRG4 is reduced in the brain of patients with Alzheimer's disease (3). In contrast, NDRG4 expression is elevated in glioblastoma and contributes to cell cycle progression and survival (8). NDRG4 also may be inactivated by promotor methylation in colorectal cancer and function as a tumor suppressor gene (9).

  1. Shimono, A. et al. (1999) Mech Dev 83, 39-52.
  2. Qu, X. et al. (2002) Mol Cell Biochem 229, 35-44.
  3. Zhou, R.H. et al. (2001) Genomics 73, 86-97.
  4. Yao, L. et al. (2008) Acta Biochim Biophys Sin (Shanghai) 40, 625-35.
  5. Ellen, T.P. et al. (2008) Carcinogenesis 29, 2-8.
  6. Yamauchi, Y. et al. (1999) Brain Res Mol Brain Res 68, 149-58.
  7. Nakada, N. et al. (2002) Brain Res Dev Brain Res 135, 45-53.
  8. Schilling, S.H. et al. (2009) J Biol Chem 284, 25160-9.
  9. Melotte, V. et al. (2009) J Natl Cancer Inst 101, 916-27.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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