Cell Signaling Technology
XP Monoclonal Antibody

Product Pathways - Neuroscience

MAG (D4G3) XP® Rabbit mAb #9043

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-F H M R Endogenous 100 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MAG (D4G3) XP® Rabbit mAb recognizes endogenous levels of total MAG protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg605 of human MAG protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, rat brain, and human cerebellum using MAG (D4G3) XP® Rabbit mAb.

IF-F

IF-F

Confocal immunofluorescent analysis of rat cerebellum using MAG (D4G3) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

Myelin-associated glycoprotein (MAG), which contains five immunoglobulin-like domains, is a highly glycosylated protein (1). MAG is a component of all myelinated internodes, whether formed by oligodendrocytes in the central nervous system (CNS) or by Schwann cells in the peripheral nervous system (PNS) (2), and has several functions. A known function of MAG is its inhibition of axonal regeneration after injury. It inhibits axonal outgrowth from adult dorsal root ganglion and in postnatal cerebellar, retinal, spinal, hippocampal, and superior cervical ganglion neurons (3). Interaction between MAG and several other molecules on the innermost wrap of myelin and complementary receptors on the opposing axon surface are required for long-term axon stability. Without MAG, myelin is still expressed, but long-term axon degeneration and altered axon cytoskeleton structure can be seen (4).

  1. Li, M. et al. (1996) J Neurosci Res 46, 404-14.
  2. Nguyen, T. et al. (2009) J Neurosci 29, 630-7.
  3. Yamashita, T. et al. (2002) J Cell Biol 157, 565-70.
  4. Mehta, N.R. et al. (2010) ACS Chem Neurosci 1, 215-222.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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