Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Podoplanin (D9D7) Rabbit mAb #9047

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Endogenous 36 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Podoplanin (D9D7) Rabbit mAb recognizes endogenous levels of total podoplanin protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp31 of human podoplanin protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using Podoplanin (D9D7) Rabbit mAb.

IP

IP

Immunoprecipitation of podoplanin from U-87 MG cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Podoplanin (D9D7) Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot analysis was performed using Podoplanin (D9D7) Rabbit mAb.

Background

Podoplanin (aggrus, glycoprotein 36) is a single-pass transmembrane protein belonging to the type-1 family of sialomucin-like glycoproteins. Podoplanin was first described in the rat as a surface glycoprotein that regulated podocyte morphology (1). It is now commonly used as a marker of lymphatic endothelial cells, where its expression is associated with the process of lymphangiogenesis (2). Its role in this regard is presumably due to its putative involvement in regulating actin cytoskeleton dynamics (3). Research studies have shown that podoplanin expression is upregulated in a number of tumor types including colorectal cancers (4), oral squamous cell carcinomas (5), and germ cell tumors (6), with higher expression levels often associated with more aggressive tumors (7). Research studies have suggested a functional role for podoplanin in the stromal microenvironment of tumors. For example, it has been reported that podoplanin expression in cancer-associated fibroblasts (CAFs) is positively associated with a stromal environment that promotes cancer progression (8,9).

  1. Breiteneder-Geleff, S. et al. (1997) Am J Pathol 151, 1141-52.
  2. Breiteneder-Geleff, S. et al. (1999) Am J Pathol 154, 385-94.
  3. Wicki, A. et al. (2006) Cancer Cell 9, 261-72.
  4. Omachi, T. et al. (2007) Cancer Lett 246, 167-72.
  5. Feng, J.Q. et al. (2012) Oral Oncol 48, 848-852.
  6. Mishima, K. et al. (2006) Acta Neuropathol 111, 563-8.
  7. Raica, M. et al. Anticancer Res 28, 2997-3006.
  8. Schoppmann, S.F. et al. (2012) Breast Cancer Res Treat 134, 237-44.
  9. Ito, M. et al. (2012) Chest 142, 151-158.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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