Cell Signaling Technology

Product Pathways - Adhesion

Phospho-CDCP1 (Tyr734) Antibody #9050

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) (R) (Mk) Endogenous 140 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-CDCP1 (Tyr734) Antibody recognizes endogenous levels of CDCP1 protein only when phosphorylated at Tyr734. This antibody also cross-reacts with an unidentified protein of 100 kDa.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr734 of human CDCP1 protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HCT 116 cells, untreated (-) or treated with Cub1 mAb (+), using Phospho-CDCP1 (Tyr734) Antibody (upper) or CDCP1 Antibody #4115 (lower).

Background

CDCP1 (CUB domain containing protein, SIMA135) is a putative stem cell marker that has been shown in research studies to be highly expressed in some human cancer cells and in both typical and atypical (cancerous) colons (1). Expression of CDCP1 may be epigenetically regulated, as methylation of promoter CpG sequences results in decreased CDCP1 expression (2). The corresponding CDCP1 gene encodes a glycoprotein that acts as a complex, multidomain transmembrane antigen. Three CUB domains in its extracellular region are possibly involved in cell adhesion or extracellular matrix interaction (1,3). Src-family kinases may phosphorylate CDCP1 at five tyrosine residues within its cytoplasmic domain to provide a potential binding site for SH2 domain-containing proteins (3). CDCP1 is a putative marker for hematopoietic stem cells (4,5).

  1. Scherl-Mostageer, M. et al. (2001) Oncogene 20, 4402-4408.
  2. Ikeda, J.I. et al. (2006) J. Pathol. 210, 75-84.
  3. Hooper, J.D. et al. (2003) Oncogene 22, 1783-1794.
  4. Conze, T. et al. (2003) Ann. N.Y. Acad. Sci. 996, 222-226.
  5. Bühring, H.J. et al. (2004) Stem Cells 22, 334-343.

Application References

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For Research Use Only. Not For Use In Diagnostic Procedures.

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