Cell Signaling Technology

Product Pathways - Neuroscience

Spinophilin Antibody #9061

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 130 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Spinophilin Antibody recognizes endogenous levels of total spinophilin protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala369 of human spinophilin protein. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various tissues and cell lines using Spinophilin Antibody.

Background

Spinophilin is an 815 amino acid protein composed of a PDZ domain, 2 actin-binding domains, a receptor- and PP1-binding domain, three coiled-coiled domains, a potential leucine/isoleucine zipper motif, and three potential SH3 domains (1). Spinophilin interacts with a large number of proteins including ion channel components and G protein-coupled receptors (GPCRs). Spinophilin also interacts with actin filaments; phosphorylation of spinophilin at Ser94 and Ser177 disrupts this interaction (2). Spinophilin has been shown to affect GPCR function through two different mechanisms: spinophilin acts as a functional inhibitor of α-2 adrenergic receptor-mediated arrestin signaling by competing with GRK2 binding to the adrenergic receptor (3) and spinophilin facilitates μ-opioid receptor desensitization by promoting receptor endocytosis (4).

  1. Sarrouilhe, D. et al. (2006) Biochimie 88, 1099-113.
  2. Hsieh-Wilson, L.C. et al. (2003) J Biol Chem 278, 1186-94.
  3. Wang, Q. et al. (2004) Science 304, 1940-4.
  4. Charlton, J.J. et al. (2008) Neuron 58, 238-47.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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