Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

RBAP46/RBAP48 (D4F8) Rabbit mAb #9067

Applications Reactivity Sensitivity MW (kDa) Isotype
W H M R Mk (Hm) (C) (GP) Endogenous 48 Rabbit IgG

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey  C=Chicken  GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RBAP46/RBAP48 (D4F8) Rabbit mAb recognizes endogenous levels of total RBAP46 and RBAP48 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RBAP48 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using RBAP46/RBAP48 (D4F8) Rabbit mAb.

Background

Retinoblastoma-associated proteins 46 and 48 (RBAP46 and RBAP48; also known as RBBP7 and RBBP4) were first characterized in human cells as proteins that bind to the retinoblastoma (Rb) tumor suppressor protein (1). Since then, these proteins have been shown to be components of many protein complexes involved in chromatin regulation, including the chromatin assembly factor 1 (CAF-1) complex and type B histone acetyltransferase complex HAT1, both of which function in chromatin assembly during DNA replication (2,3). RBAP46 and RBAP48 are also found in the nucleosome remodeling factor complex NURF, the nucleosome remodeling and histone de-acetylation complex NuRD, and the Sin3/HDAC histone de-acetylation complex (4-7). More recently, RBAP46 and RBAP48 were identified as components of the polycomb repressor complex PRC2, which also contains EED and Ezh2 (8). RBAP46 and RBAP48 bind to the histone fold region of histone H4 and are believed to target these chromatin remodeling, histone acetylation, and histone de-acetylation complexes to their histone substrates (3).

  1. Qian, Y.W. et al. (1993) Nature 364, 648-52.
  2. Verreault, A. et al. (1996) Cell 87, 95-104.
  3. Verreault, A. et al. (1998) Curr Biol 8, 96-108.
  4. Barak, O. et al. (2003) EMBO J 22, 6089-100.
  5. Zhang, Y. et al. (1999) Genes Dev 13, 1924-35.
  6. Zhang, Y. et al. (1997) Cell 89, 357-64.
  7. Kuzmichev, A. et al. (2002) Genes Dev 16, 2893-905.
  8. Cao, R. et al. (2002) Science 298, 1039-43.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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