Cell Signaling Technology

Product Pathways - Neuroscience

STEP (D9H3) Rabbit mAb #9069

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Endogenous 46, 61 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

STEP (D9H3) Rabbit mAb detects endogenous levels of total STEP46 and STEP61 proteins.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ile440 of human STEP61 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse and rat brain using STEP (D9H3) Rabbit mAb.

Background

Striatal enriched phosphatase (STEP, also known as PTPN5), is a protein tyrosine phosphatase expressed in dopaminoceptive neurons of the central nervous system (1). Alternative splicing produces the cytosolic STEP46 and the membrane-associated STEP61 isoforms of STEP. Dopamine activates D1 receptors and PKA, which in turn phosphorylate both isoforms of STEP. Phosphorylation of STEP61 occurs at Ser160 and Ser221, while STEP46 is phosphorylated at Ser49 (equivalent to Ser221 of STEP61) (2). NMDA-mediated activation of STEP is an important mechanism for regulation of Erk activity in neurons (3). Furthermore, STEP is involved in the regulation of both NMDAR and AMPAR trafficking (4,5). Due to its importance in cognitive function, STEP may play a role in Alzheimer's disease (1).

  1. Braithwaite, S.P. et al. (2006) Trends Neurosci 29, 452-8.
  2. Paul, S. et al. (2000) J Neurosci 20, 5630-8.
  3. Paul, S. et al. (2003) Nat Neurosci 6, 34-42.
  4. Braithwaite, S.P. et al. (2006) Eur J Neurosci 23, 2847-56.
  5. Zhang, Y. et al. (2008) J Neurosci 28, 10561-6.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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