Buffers
ProLong® Gold Antifade Reagent #9071
Directions for Use
Following immunostaining, cover samples with ProLong® Gold Antifade Reagent and coverslip.
To prevent fading, allow slides to cure in the dark, at room temperature for 24 hours, then seal the coverslips with clear lacquer, such as nail polish.
Proceed with imaging.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using β-Actin (8H10D10) Mouse mAb #3700 (red) and COX IV (3E11) Rabbit mAb (Alexa Fluor® 488 Conjugate) #4853 (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
IF-IC
Confocal immunofluorescent analysis of COS-7 cells, mounted in either glycerol (upper panels) or ProLong® Gold Antifade Reagent (lower panels), using β-Actin (8H10D10) Mouse mAb #3700 and Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 555 Conjugate) #4409 (red) (0, 3.3, or 10 min of imaging).
IF-IC
Confocal immunofluorescent analysis of SNB19 cells using β-Actin (8H10D10) Mouse mAb #3700 (red), Vimentin (D21H3) XP® Rabbit mAb (Alexa Fluor® 488 Conjugate) #9854 (green), and COX IV (3E11) Rabbit mAb (Alexa Fluor® 647 Conjugate) #7561 (blue).
Background
ProLong® Gold Antifade Reagent offers enhanced resistance to photobleaching, is premixed and ready to use, and causes little or no quenching of the fluorescent signal. The reagent cures within 24 hours and samples can be saved for months after mounting. This reagent is compatible with most dyes and is appropriate for use in multi-color labeling assays.
Application References
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The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
