Product Pathways - Companion Products
MitoTracker® Green FM #9074
|9074S||500 µg (10 x 50 ug)||---||In Stock||---|
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MitoTracker® Green FM is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 490 nm, Emission: 516 nm, Molecular Weight: 671.88 g/mol
MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.
Directions For Use
Each vial of MitoTracker® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX!
To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging. BackDrop® Green Background Suppressor #12388 can also be added to reduce background fluorescence.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
BackDrop® is a registered trademark of Molecular Probes, Inc.
MitoTracker® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.