Cellular Dyes
MitoTracker® Green FM #9074
Directions for Use
Each vial of MitoTracker® Green FM contains 50 µg lyophilized solid. To make a 1 mM stock solution, reconstitute the solid in 74.4 µl high quality DMSO. While the optimal staining concentration and incubation times can vary by application, typical results are obtained by diluting the stock solution directly into normal growth media at a concentration between 100-400 nM and incubating between 15-30 min at 37ºC. After incubation, the cells must be imaged live; DO NOT FIX!
To reduce background fluorescence of the media, phenol-red free media can be substituted for normal media prior to imaging. A green background suppressor, such as BackDrop™ Background Suppressor from Life Technologies, can also be added to reduce background fluorescence.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using MitoTracker® Green FM. Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Description
MitoTracker® Green FM is recommended for live cell imaging only; fixation with aldehydes or alcohols will inhibit staining. Excitation: 490 nm, Emission: 516 nm, Molecular Weight: 671.88
Background
MitoTracker® dyes are cell permeable probes that contain a mildly thiol-reactive chloromethyl moiety for mitochondrial labeling. To label mitochondria, cells are simply incubated with MitoTracker® probes, which passively diffuse across the plasma membrane and accumulate in active mitochondria.
Application References
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The transfer of this product is contingent on the buyer using the purchased product solely in research conducted by the buyer (whether the buyer is an academic or for-profit entity), for immunocytochemistry, high content screening (HCS) analysis, or flow cytometry applications. The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) resale, whether or not such product or its components are resold for use in research; or for any other commercial purpose. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. MitoTracker® is a registered trademark of Molecular Probes, Inc.
For Research Use Only. Not For Use In Diagnostic Procedures.
