Product Pathways - Companion Products
Rabbit (DA1E) mAb IgG XP® Isotype Control (Pacific Blue™ Conjugate) #9078
| Applications | Reactivity | Isotype |
|---|---|---|
| F | Rabbit IgG |
Applications Key:
F=Flow Cytometry
Reactivity Key:
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 9078:
- Flow
Specificity / Sensitivity
Rabbit (DA1E) mAb IgG XP® Isotype Control (Pacific Blue™ Conjugate) is not directed against any known antigen. It functions as an isotype control for rabbit IgG monoclonal antibodies.
Source / Purification
Flow Cytometry
Flow cytometric analysis of Jurkat cells, untreated (green) or treated with LY294002 #9901, Wortmannin #9951, and U0126 #9903 (blue) using Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP® Rabbit mAb (Pacific Blue™ Conjugate) #8520 compared to Rabbit (DA1E) mAb IgG XP® Isotype Control (Pacific Blue™ Conjugate) (red).
Description
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometry analysis in human cells.
Background
Isotype control antibodies are used to estimate the nonspecific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
For Research Use Only. Not For Use In Diagnostic Procedures.
